If the media tubes are damaged or missing something in the packaging can we get a replacement?

Please request fresh tubes on our website (Vet Portal > Request Supplies).  

If you need to send cells immediately, please use the serum and saline protocol from the CSU Hemapathology Lab:

  1. Place 1ml of saline (.9%, LRS, Norm R) into a no-additive, white-top or red-top tube (plain tube with no additives or serum separator).
  2. Add .1 ml of serum from the patient, or another healthy animal of the same species, to the saline in the white-top or red-top tube.
  3. Aspirate using suction.Gently squirt contents into the tube containing the saline and serum mixture.
  4. Rinse syringe by drawing up saline and serum mixture and gently squirting it back into the tube. Don’t make bubbles.
  5. Repeat aspiration and rinsing multiple times until solution is no longer transparent. A turbid solution usually indicates adequate cellularity for flow cytometry testing.

Other Questions

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What is included in the ImpriMed Immunoprofile service?

The ImpriMed Immunoprofile service includes both our Flow Cytometry and PARR reports. These comprehensive results can be used to detect the presence of lymphoma or leukemia and determine the disease subtype.

I have a patient with likely lymphoma/leukemia. For a blood sample, can I just submit an EDTA blood tube or do I still use the ImpriMed tubes (and if so, how many tubes and is an additional EDTA blood tube still needed with that)?

If the patient is suspected of leukemia, blood collected in the EDTA tube should be fine. If it is lymphoma, we might need FNA from the affected organ/lymph node. We can suggest FNA in ImpriMed media tube (1) and 2ml of blood sample in an EDTA tube.

What does PARR tell me about my patient’s specimen?

PARR, which stands for PCR for Antigen Receptor Rearrangements, is used to discriminate between lymphoma/leukemia and reactive/inflammatory conditions when cytology is equivocal. Our canine PARR assay detects the expansion of B-cell cancer clones by amplifying the VJ region of the immunoglobulin heavy chain gene (IgH) and detects the expansion of T-cell cancer clones by amplifying a region in the T-cell receptor gamma chain gene.

Can the test be used on cats and other animals?

At this time, we only offer commercial services to canine lymphoma patients. However, we are conducting research into feline lymphoma so if you would like to submit a sample please sign up here if you are interested in feline services.

How much blood is required for Personalized Prediction Profile for Canine Lymphoma?

We require at least 2mL of whole blood sample in an EDTA-treated tube.