How do I prepare serum and saline tube?

If you do not have our proprietary media tubes handy at the moment, please use the serum and saline protocol from the CSU Hemapathology Lab:

  1. Place 1ml of saline (.9%, LRS, Norm R) into a no-additive, white-top or red-top tube (plain tube with no additives or serum separator).
  2. Add .1 ml of serum from the patient, or another healthy animal of the same species, to the saline in the white-top or red-top tube.Aspirate using suction.Gently squirt contents into the tube containing the saline and serum mixture.
  3. Rinse syringe by drawing up saline and serum mixture and gently squirting it back into the tube. Don’t make bubbles.
  4. Repeat aspiration and rinsing multiple times until solution is no longer transparent. A turbid solution usually indicates adequate cellularity for flow cytometry testing.

Other Questions

Back to Help Center

What does the ImpriMed Personalized Prediction Profile include?

The Personalized Prediction Profile includes our Immunoprofile report and anticancer drug response predictions generated by artificial intelligence models. The predictions include estimates of both (1) the likelihood of a positive clinical response (partial response/ complete remission) to individual anticancer drugs and (2) the likelihood complete remission after 1 or 2 cycles of CHOP therapy, and the likelihood of early relapse after a successful CHOP regimen. Outcome predictions are currently provided for 13 individual drugs that are commonly used in many first-line and rescue protocols for the treatment of canine lymphoma (including CHOP, LOPP, MOPP, LPP, Tavonea only, etc…). Predictions are included for Doxorubicin, Cyclophosphamide, Vincristine, Vinblastine, Prednisone, Rabacfosadine (Tanovea®), L-Asparaginase, Lomustine, Mitoxantrone, Mechlorethamine, Dexamethasone, Chlorambucil, and Melphalan.

Read More

Do you need me to provide you with passwords for the hospital accounts?

No, we don’t need the password to associate the doctors and hospital. Once we register it, the password will be sent to the corresponding emails.

Read More

Do you test "rescue" drugs as well?

Yes, many of the 13 anticancer drugs we provide predictions for are used for rescue therapy. The drugs we test against are: L-Asparaginase, Mitoxantrone, Vincristine, Vinblastine, Doxorubicin, Tanovea, Chlorambucil, Mechlorethamine, Lomustine, Prednisone, Cyclophosphamide, Melphalan, and Dexamethasone.

Read More

We have a case coming in today we would really like to submit but don’t have transport media tubes; is there any other method we could do in order to submit a sample, such as aspirating and placing it in tube with serum?

Please use serum and saline that you would normally do when you send to other reference laboratories.

Read More

How does ImpriMed predict drug responses?

Our predictions are made by artificial intelligence (AI) models trained to predict clinical outcomes from patient data inputs. Clinical outcomes collected from oncologists include reports of progressive disease, stable disease, partial response, and complete response. Patient data used as inputs by the AI models include readings from our live-cell drug sensitivity assay, flow cytometry, PARR, and patient information.Models are re-trained periodically to incorporate new data and refine performance.

Read More