How do I prepare serum and saline tube?

If you do not have our proprietary media tubes handy at the moment, please use the serum and saline protocol from the CSU Hemapathology Lab:

1. Place 1ml of saline (.9%, LRS, Norm R) into a no-additive, white-top or red-top tube (plain tube with no additives or serum separator).
2. Add .1 ml of serum from the patient, or another healthy animal of the same species, to the saline in the white-top or red-top tube.Aspirate using suction.Gently squirt contents into the tube containing the saline and serum mixture.
3. Rinse syringe by drawing up saline and serum mixture and gently squirting it back into the tube. Don’t make bubbles.
4. Repeat aspiration and rinsing multiple times until solution is no longer transparent. A turbid solution usually indicates adequate cellularity for flow cytometry testing.