How do I prepare serum and saline tube?

If you do not have our proprietary media tubes handy at the moment, please use the serum and saline protocol from the CSU Hemapathology Lab:

  1. Place 1ml of saline (.9%, LRS, Norm R) into a no-additive, white-top or red-top tube (plain tube with no additives or serum separator).
  2. Add .1 ml of serum from the patient, or another healthy animal of the same species, to the saline in the white-top or red-top tube.Aspirate using suction.Gently squirt contents into the tube containing the saline and serum mixture.
  3. Rinse syringe by drawing up saline and serum mixture and gently squirting it back into the tube. Don’t make bubbles.
  4. Repeat aspiration and rinsing multiple times until solution is no longer transparent. A turbid solution usually indicates adequate cellularity for flow cytometry testing.

Other Questions

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How can I help to advance precision medicine?

The more samples ImpriMed processes and the more clinical outcomes we receive from our customers, the more accurately our AI models are able to determine which drugs will work best for your patients. (Click to read more)

If the media tubes are damaged or missing something in the packaging can we get a replacement?

Please request fresh tubes on our website (Vet Portal > Request Supplies). If you need to send cells immediately, please use the serum and saline protocol from the CSU Hemapathology Lab. (Click to read more)

I have a patient with likely lymphoma/leukemia. For a blood sample, can I just submit an EDTA blood tube or do I still use the ImpriMed tubes (and if so, how many tubes and is an additional EDTA blood tube still needed with that)?

If the patient is suspected of leukemia, blood collected in the EDTA tube should be fine. If it is lymphoma, we might need FNA from the affected organ/lymph node. We can suggest FNA in ImpriMed media tube (1) and 2ml of blood sample in an EDTA tube.

What does the ImpriMed Personalized Prediction Profile include?

The Personalized Prediction Profile includes our Immunoprofile report and anticancer drug response predictions generated by artificial intelligence models. The predictions include estimates of both (1) the likelihood of a positive clinical response (partial response/ complete remission) to individual anticancer drugs and (2) the likelihood complete remission after 1 or 2 cycles of CHOP therapy, and the likelihood of early relapse after a successful CHOP regimen. Outcome predictions are currently provided for 13 individual drugs that are commonly used in many first-line and rescue protocols for the treatment of canine lymphoma (including CHOP, LOPP, MOPP, LPP, Tavonea only, etc…). Predictions are included for Doxorubicin, Cyclophosphamide, Vincristine, Vinblastine, Prednisone, Rabacfosadine (Tanovea®), L-Asparaginase, Lomustine, Mitoxantrone, Mechlorethamine, Dexamethasone, Chlorambucil, and Melphalan.

Which cancers can I use ImpriMed for? or What type of cancer does ImpriMed predict?

Currently, our services are for canine lymphoma and leukemia. We will be expanding our services to other species and other blood / lymphoid cancers in the near future.