Media tubes and sample preparation

Learn what you need to collect and send

When can I expect to receive the sample return boxes I requested online?

You can expect to receive the boxes in 7-12 days via FedEx Ground.

For patients who already have flow cytometry, can you just run the drug sensitivity profile?

No, we cannot run a drug sensitivity-only service. Please understand our AI-based drug response prediction models require flow cytometry and PARR parameters generated from our own instrumental setting. Even if the patient already has flow cytometry results from another laboratory, we need to run flow and PARR again anyway.

How should I discard the expired media we still have?

The media and tubes can be decontaminated in or with 10% bleach and discarded as normal.

I have a leukemia patient. Can you run an assay on blood?

Yes, we do run assays on blood for a leukemia patient. Please send us at least 2 mL of whole blood in an EDTA tube.

If the media tubes are damaged or missing something in the packaging can we get a replacement?

Please request fresh tubes on our website (Vet Portal > Request Supplies). If you need to send cells immediately, please use the serum and saline protocol from the CSU Hemapathology Lab. (Click to read more)

How long can the media tubes be outside of that temperature before going bad?

We will send you another batch of media tubes you could use. Please make the supply request on the Vet Portal > Request Supplies page.

How much blood is required for Personalized Prediction Profile for Canine Lymphoma?

We require at least 2mL of whole blood sample in an EDTA-treated tube.

For the blood tube submission– Do you request that on every patient, even if they don't have a leukemic component? And what type of blood tube do you request we send the sample in?

We require at least 2 mL of whole blood in an EDTA-treated tube.

What type of sample is needed – just aspirates and a typical flow sample?

We need about 10 million cancer cells to run the full chemosensitivity panel so we ask that you conduct an aggressive woodpecker style FNA, poking as many nodes as possible and putting the cells into our proprietary media tubes (ensures we receive live cells), then of course we need a whole blood sample (2mL).

I put the media tubes into the freezer. Can I still use the media tubes?

No, We will send you another batch of media tubes you could use. Please email us at support@imprimedicine.com or request fresh tubes online via our Vet Portal.

What temperature do the media tubes need to be stored at?

Our proprietary media tubes must be stored in the fridge (36-40° F) before use. Please DO NOT freeze the media tubes.

How long does the media last before expiring? How long is the medium good for?

The media has a 3 month shelf life. You can check the tube and look for its expiration date as well.

Can the blood sample be frozen or refrigerated before shipping?

The blood sample can be refrigerated before shipping. Please DO NOT freeze the blood sample.

How do we ship?

Please follow these steps to prepare the box and ship the sample to our lab. (Click to read more)

How do I collect an FNA sample?

Disinfect the skin with alcohol (70%) at the planned needle puncture site. Insert a 20-gauge needle (without syringe) into an enlarged lymph node and perform 15 - 20 aspirations using an aggressive “woodpecker style” technique. (Click to Read More)

How do I prepare serum and saline tube?

If you do not have our proprietary media tubes handy at the moment, please use the serum and saline protocol from the CSU Hemapathology Lab

We have a case coming in today we would really like to submit but don’t have transport media tubes; is there any other method we could do in order to submit a sample, such as aspirating and placing it in tube with serum?

Please use serum and saline that you would normally do when you send to other reference laboratories.

I have a patient with likely lymphoma/leukemia. For a blood sample, can I just submit an EDTA blood tube or do I still use the ImpriMed tubes (and if so, how many tubes and is an additional EDTA blood tube still needed with that)?

If the patient is suspected of leukemia, blood collected in the EDTA tube should be fine. If it is lymphoma, we might need FNA from the affected organ/lymph node. We can suggest FNA in ImpriMed media tube (1) and 2ml of blood sample in an EDTA tube.

Can the testing be performed on dogs that are currently on therapy?

Current therapy will not affect our AI predictions or immunoprofile results. However, reduction of tumor size caused by therapy may increase the likelihood of service failure due to insufficient cells. In the event of service failure, you will not be billed.

If the blood clots can the ImpriMed test still be run?

Blood clots can prevent our ability to run our tests. Very small clots may not be a problem, but in general, clotted blood is not ideal. That is why we ask that doctors send blood in an EDTA or heparin tube.

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